Recombinant:Article Title: Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in vivo or in vitro experimentation.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Dulbecco’s modified Eagle’s medium (DMEM) (alternatives available) Corning Cat#10-013-CV 1 M 2-(N-morpholino)ethanesulfonic acid (MES) MilliporeSigma Cat#M1317 Polyethylene glycol (PEG), 50% (w/v) solution Fisher Scientific Cat#AAJ61495AE 3-((3-Cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS) powder (alternatives available) Fisher Scientific Cat#BP571-1 Brij 35, 30% (w/w) aqueous wetting agent (alternatives available) Ricca Chemical Cat#1145-16 1 M Tris hydrochloride solution, pH 7.5 (alternatives available) Fisher Scientific Cat#BP1757-100 Recombinant human MMP-12 (rhMMP-12) R&D Systems Cat#917-MPB Recombinant mouse MMP-12 (rmMMP-12) R&D Systems Cat#3467-MPB 10 mM p-aminophenylmercuric acetate MedChemExpress Cat#HY-148905 LaRee 5 powder SiChem CatSC-0043 Recombinant mouse TIMP-1 protein R&D Systems Cat#980-MT Experimental models: Organisms/strains Mouse: C57BL/6J, 12–14 weeks, male and female Jackson Laboratory Cat#000664 Software and algorithms Analyze and Acquire software, version 3.0.0.53 Spectradyne https://nanoparticleanalyzer.com/ dlhidgen3/ Prism software, version 10.3.1 GraphPad https://www.graphpad.com/ Cary UV workstation software Agilent https://www.agilent.com/en/product/ molecular-spectroscopy/uv-vis-uvvis-nir-spectroscopy/uv-vis-uv-visnir-software/cary-uv-workstation-software (Continued on next page) 6 STAR Protocols 6, 103867, June 20, 2025 MATERIALS AND EQUIPMENT .. SoftMax Pro 7 data acquisition and analysis software, version 7.1.2 Molecular Devices https://www.moleculardevices.com/ products/microplate-readers/ acquisition-and-analysis-software/ softmax-pro-software Other 500 mL 0.2 μm filter bottle (alternatives available) Fisher Scientific Cat#FB12566504 0.2 μm syringe filters (alternatives available) Fisher Scientific Cat#09-928-062 0.02 μm syringe filter, 25 mm diameter (alternatives available) Fisher Scientific Cat#09-926-13 25 3 89 mm open-top polyallomer tubes Fisher Scientific Cat#03-141 8 3 51 mm open-top polyallomer tubes Seton Scientific Cat#5009 Amicon 4 mL centrifugal filter, 100 kDa MWCO MilliporeSigma Cat#UFC810024 Amicon 0.5 mL centrifugal filter, 10 kDa MWCO MilliporeSigma Cat#UFC501096 1 mL Luer slip tip syringe Fisher Scientific Cat#14-955-462 10 mL Luer-Lok syringe Fisher Scientific Cat#B302995 20 mL Luer-Lok syringe Fisher Scientific Cat#22-124-967 50 mL Luer-Lok syringe Fisher Scientific Cat#13-689-8 Insyte Autoguard 20-guage catheter Becton Dickinson Cat#381434 Mouse dissecting kit World Precision Instruments Cat#MOUSEKIT 2 in segment of Tygon S3 E-3603 laboratory tubing, inner diameter 3/8 in DWK Life Sciences Cat#869791-0038 3-way stopcock Fisher Scientific Cat#NC9779127 Parafilm M self-sealing film Fisher Scientific Cat#STLPM996 6-in length forceps with serrated tip (alternatives available) Fisher Scientific Cat#16-100-108 Research cigarette, 3R4F University of Kentucky Cat#3R4F Semi-micro spectrophotometer cell Starna Cells Cat#9/9-Q-10 24-well tissue culture plate Fisher Scientific Cat#08-772-1 200 μL extended length pipette tips Corning Cat#4810 Microfluidic cartridges Spectradyne Cat#C-400 96-well half-area, black, flat bottom polystyrene microplate Corning Cat#3993 Automated cell counter DeNovix Model#CellDropFLi Cary 60 UV-vis spectrophotometer Agilent Model#G6860AA Sorvall WX+ ultracentrifuge Fisher Scientific Cat#75-000-080 AH-629 swinging bucket rotor Fisher Scientific Cat#54-285 TFT-80.2 fixed angle rotor Fisher Scientific Cat#54-456 Nanoparticle analyzer Spectradyne Model#nCS1 SpectraMax multi-mode microplate reader Molecular Devices Model#iD5 rhMMP-12 reconstitution buffer
Modification:Article Title: Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in vivo or in vitro experimentation.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Dulbecco’s modified Eagle’s medium (DMEM) (alternatives available) Corning Cat#10-013-CV 1 M 2-(N-morpholino)ethanesulfonic acid (MES) MilliporeSigma Cat#M1317 Polyethylene glycol (PEG), 50% (w/v) solution Fisher Scientific Cat#AAJ61495AE 3-((3-Cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS) powder (alternatives available) Fisher Scientific Cat#BP571-1 Brij 35, 30% (w/w) aqueous wetting agent (alternatives available) Ricca Chemical Cat#1145-16 1 M Tris hydrochloride solution, pH 7.5 (alternatives available) Fisher Scientific Cat#BP1757-100 Recombinant human MMP-12 (rhMMP-12) R&D Systems Cat#917-MPB Recombinant mouse MMP-12 (rmMMP-12) R&D Systems Cat#3467-MPB 10 mM p-aminophenylmercuric acetate MedChemExpress Cat#HY-148905 LaRee 5 powder SiChem CatSC-0043 Recombinant mouse TIMP-1 protein R&D Systems Cat#980-MT Experimental models: Organisms/strains Mouse: C57BL/6J, 12–14 weeks, male and female Jackson Laboratory Cat#000664 Software and algorithms Analyze and Acquire software, version 3.0.0.53 Spectradyne https://nanoparticleanalyzer.com/ dlhidgen3/ Prism software, version 10.3.1 GraphPad https://www.graphpad.com/ Cary UV workstation software Agilent https://www.agilent.com/en/product/ molecular-spectroscopy/uv-vis-uvvis-nir-spectroscopy/uv-vis-uv-visnir-software/cary-uv-workstation-software (Continued on next page) 6 STAR Protocols 6, 103867, June 20, 2025 MATERIALS AND EQUIPMENT .. SoftMax Pro 7 data acquisition and analysis software, version 7.1.2 Molecular Devices https://www.moleculardevices.com/ products/microplate-readers/ acquisition-and-analysis-software/ softmax-pro-software Other 500 mL 0.2 μm filter bottle (alternatives available) Fisher Scientific Cat#FB12566504 0.2 μm syringe filters (alternatives available) Fisher Scientific Cat#09-928-062 0.02 μm syringe filter, 25 mm diameter (alternatives available) Fisher Scientific Cat#09-926-13 25 3 89 mm open-top polyallomer tubes Fisher Scientific Cat#03-141 8 3 51 mm open-top polyallomer tubes Seton Scientific Cat#5009 Amicon 4 mL centrifugal filter, 100 kDa MWCO MilliporeSigma Cat#UFC810024 Amicon 0.5 mL centrifugal filter, 10 kDa MWCO MilliporeSigma Cat#UFC501096 1 mL Luer slip tip syringe Fisher Scientific Cat#14-955-462 10 mL Luer-Lok syringe Fisher Scientific Cat#B302995 20 mL Luer-Lok syringe Fisher Scientific Cat#22-124-967 50 mL Luer-Lok syringe Fisher Scientific Cat#13-689-8 Insyte Autoguard 20-guage catheter Becton Dickinson Cat#381434 Mouse dissecting kit World Precision Instruments Cat#MOUSEKIT 2 in segment of Tygon S3 E-3603 laboratory tubing, inner diameter 3/8 in DWK Life Sciences Cat#869791-0038 3-way stopcock Fisher Scientific Cat#NC9779127 Parafilm M self-sealing film Fisher Scientific Cat#STLPM996 6-in length forceps with serrated tip (alternatives available) Fisher Scientific Cat#16-100-108 Research cigarette, 3R4F University of Kentucky Cat#3R4F Semi-micro spectrophotometer cell Starna Cells Cat#9/9-Q-10 24-well tissue culture plate Fisher Scientific Cat#08-772-1 200 μL extended length pipette tips Corning Cat#4810 Microfluidic cartridges Spectradyne Cat#C-400 96-well half-area, black, flat bottom polystyrene microplate Corning Cat#3993 Automated cell counter DeNovix Model#CellDropFLi Cary 60 UV-vis spectrophotometer Agilent Model#G6860AA Sorvall WX+ ultracentrifuge Fisher Scientific Cat#75-000-080 AH-629 swinging bucket rotor Fisher Scientific Cat#54-285 TFT-80.2 fixed angle rotor Fisher Scientific Cat#54-456 Nanoparticle analyzer Spectradyne Model#nCS1 SpectraMax multi-mode microplate reader Molecular Devices Model#iD5 rhMMP-12 reconstitution buffer
Software:Article Title: Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in vivo or in vitro experimentation.
Article Snippet: .. Chemicals, peptides, and recombinant proteins Dulbecco’s modified Eagle’s medium (DMEM) (alternatives available) Corning Cat#10-013-CV 1 M 2-(N-morpholino)ethanesulfonic acid (MES) MilliporeSigma Cat#M1317 Polyethylene glycol (PEG), 50% (w/v) solution Fisher Scientific Cat#AAJ61495AE 3-((3-Cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS) powder (alternatives available) Fisher Scientific Cat#BP571-1 Brij 35, 30% (w/w) aqueous wetting agent (alternatives available) Ricca Chemical Cat#1145-16 1 M Tris hydrochloride solution, pH 7.5 (alternatives available) Fisher Scientific Cat#BP1757-100 Recombinant human MMP-12 (rhMMP-12) R&D Systems Cat#917-MPB Recombinant mouse MMP-12 (rmMMP-12) R&D Systems Cat#3467-MPB 10 mM p-aminophenylmercuric acetate MedChemExpress Cat#HY-148905 LaRee 5 powder SiChem CatSC-0043 Recombinant mouse TIMP-1 protein R&D Systems Cat#980-MT Experimental models: Organisms/strains Mouse: C57BL/6J, 12–14 weeks, male and female Jackson Laboratory Cat#000664 Software and algorithms Analyze and Acquire software, version 3.0.0.53 Spectradyne https://nanoparticleanalyzer.com/ dlhidgen3/ Prism software, version 10.3.1 GraphPad https://www.graphpad.com/ Cary UV workstation software Agilent https://www.agilent.com/en/product/ molecular-spectroscopy/uv-vis-uvvis-nir-spectroscopy/uv-vis-uv-visnir-software/cary-uv-workstation-software (Continued on next page) 6 STAR Protocols 6, 103867, June 20, 2025 MATERIALS AND EQUIPMENT .. SoftMax Pro 7 data acquisition and analysis software, version 7.1.2 Molecular Devices https://www.moleculardevices.com/ products/microplate-readers/ acquisition-and-analysis-software/ softmax-pro-software Other 500 mL 0.2 μm filter bottle (alternatives available) Fisher Scientific Cat#FB12566504 0.2 μm syringe filters (alternatives available) Fisher Scientific Cat#09-928-062 0.02 μm syringe filter, 25 mm diameter (alternatives available) Fisher Scientific Cat#09-926-13 25 3 89 mm open-top polyallomer tubes Fisher Scientific Cat#03-141 8 3 51 mm open-top polyallomer tubes Seton Scientific Cat#5009 Amicon 4 mL centrifugal filter, 100 kDa MWCO MilliporeSigma Cat#UFC810024 Amicon 0.5 mL centrifugal filter, 10 kDa MWCO MilliporeSigma Cat#UFC501096 1 mL Luer slip tip syringe Fisher Scientific Cat#14-955-462 10 mL Luer-Lok syringe Fisher Scientific Cat#B302995 20 mL Luer-Lok syringe Fisher Scientific Cat#22-124-967 50 mL Luer-Lok syringe Fisher Scientific Cat#13-689-8 Insyte Autoguard 20-guage catheter Becton Dickinson Cat#381434 Mouse dissecting kit World Precision Instruments Cat#MOUSEKIT 2 in segment of Tygon S3 E-3603 laboratory tubing, inner diameter 3/8 in DWK Life Sciences Cat#869791-0038 3-way stopcock Fisher Scientific Cat#NC9779127 Parafilm M self-sealing film Fisher Scientific Cat#STLPM996 6-in length forceps with serrated tip (alternatives available) Fisher Scientific Cat#16-100-108 Research cigarette, 3R4F University of Kentucky Cat#3R4F Semi-micro spectrophotometer cell Starna Cells Cat#9/9-Q-10 24-well tissue culture plate Fisher Scientific Cat#08-772-1 200 μL extended length pipette tips Corning Cat#4810 Microfluidic cartridges Spectradyne Cat#C-400 96-well half-area, black, flat bottom polystyrene microplate Corning Cat#3993 Automated cell counter DeNovix Model#CellDropFLi Cary 60 UV-vis spectrophotometer Agilent Model#G6860AA Sorvall WX+ ultracentrifuge Fisher Scientific Cat#75-000-080 AH-629 swinging bucket rotor Fisher Scientific Cat#54-285 TFT-80.2 fixed angle rotor Fisher Scientific Cat#54-456 Nanoparticle analyzer Spectradyne Model#nCS1 SpectraMax multi-mode microplate reader Molecular Devices Model#iD5 rhMMP-12 reconstitution buffer
|
R&D Systems is a verified supplier 
R&D Systems manufactures this product 